Method for Diagnosing Diseases Based on Levels of Anti-Glycan Antibodies

ABSTRACT

Disclosed are methods for diagnosing Crohn&#39;s disease (CD) or anti-phospholipid syndrome by measuring levels of antibodies to glycans in a biological sample.

RELATED APPLICATIONS

This application is a continuation of U.S. Ser. No. 10/728,227, filed Dec. 3, 2003 (pending), the contents of which are incorporated by reference herein in their entirety.

FIELD OF THE INVENTION

The invention relates generally to a method for diagnosing diseases by detecting levels of anti-bodies to glycans in a subject. More particularly, the invention relates to methods for diagnosing Crohn's disease (CD) or anti-phospholipid syndrome (APS).

BACKGROUND OF THE INVENTION

Inflammatory bowel disease (IBD), which occurs world-wide and afflicts millions of people, is the collective term used to describe two gastrointestinal disorders of unknown etiology: Crohn's disease (CD) and ulcerative colitis (UC). IBD and irritable bowel syndrome (IBS) will affect one-half of all Americans during their lifetime, at a cost of several billion dollars. A primary determinant of these high medical costs is the difficulty of diagnosing digestive diseases. The cost associated with IBD and IBS is compounded by lost productivity, with persons suffering from these disorders missing at least 8 more days of work annually than the national average.

Symptoms associated with Crohn's disease include, e.g., abdominal pain, chronic diarrhea, rectal bleeding, weight loss and cramping. These symptoms are also found in common with irritable bowel syndrome and other inflammatory bowel diseases. This makes definitive diagnosis of CD extremely difficult. In fact, only about one-tenth of the several million people suspected of suffering from CD are actually diagnosed with the disease The difficulty in differentially diagnosing CD from other digestive diseases like UC and IBS hampers early and effective treatment of these diseases.

Crohn's disease (ileitis regionalis or ileitis terminalis) may affect any part of the gut with the ileum and colon as the most commonly affected sites. In CD the inflammation is asymmetrical and segmental, with areas of both healthy and diseased tissue. By contrast, ulcerative colitis (hemorrhagic idiopathic proctocolitis) is characterized by symmetrical inflammation-restricted to mucosa and submucosa—ascending uninterrupted from rectum to colon.

Crohn's disease is typically diagnosed using upper or lower GI endoscopy and/or by X-ray examination of the small intestine including ileum. In CD no typical endoscopic picture is shown, while in UC the typical pattern detected is an inflamed red mucosal with bleeding. In CD biopsy specimens reveal transmural inflammation with lymphocytes, macrophages and plasma cells while mucosal/submucosal inflammation with granulocytes, eosinophiles and plasma cells are typical findings in UC.

Antiphospholipid syndrome (APS) is characterized by venous or arterial thrombosis, recurrent miscarriages, and thrombocytopenia, which is a low number of blood platelets that can lead to bleeding, seen as bruising and tiny red dots on the skin. Patients with APS also may experience symptoms of stroke such as transient ischemic attacks (TIAs).

Antiphospholipid syndrome is typically diagnosed based on these clinical manifestations and on laboratory test results. A blood sample is analyzed for the presence of antibodies that react with naturally occurring proteins complexed with phospholipids. These are called antiphospholipid antibodies or anticardiolipin antibodies (cardiolipin is one type of phospholipid used in lab tests). Sometimes these antibodies are called lupus anticoagulants when clotting assays are used for their detection.

SUMMARY OF THE INVENTION

The invention is based in part on the discovery that patients with Crohn's disease (CD) or anti-phospholipid syndrome (APS) have elevated serum levels of certain IgG, IgA, and IgM isotype antibodies specific for certain glycan structures, as compared to as compared to the serum levels of these antibodies in healthy individuals or in individuals with other types of gastrointestinal diseases.

Among the advantages of the invention is a highly sensitive serological testing method for definitively distinguishing CD from other digestive diseases. The highly sensitive primary screening assays according to the invention provide physicians with an inexpensive assay for rapidly distinguishing individuals with CD from non diseased individuals, or individuals having UC or IBS. This facilitates earlier and more appropriate therapeutic intervention and minimizing uncertainty for patients and their families.

In one aspect, the invention provides a method of diagnosing Crohn's disease in a subject by providing a test sample from the subject and detecting in the test sample at least one of the following anti-glycan antibodies: anti-Glc (β) antibody, an anti-Glc (β 1-4) Glc (β) antibody, an anti-Glc (β 1-3) Glc (β) antibody, an anti-GlcNAc 6-sulfate (β) antibody an anti-Dextran antibody, an anti-Xylan antibody, an anti-GlcNAc (β 1-4) GlcNAc (β) antibody, an anti-Gal 3 sulphate (β) antibody, an anti-GlcNAc (β 1-3) GalNAc (β) antibody, an anti-GlcNAc (β 1-3) Gal (β 1-4) Glc (β) antibody, and an anti-Gal (α 1-3) Gal (β 1-4) GlcNAc (β) antibody. The presence of one or more of the antibodies in the test sample indicates the subject has Crohn's disease.

In some embodiments, levels of the anti-glycan antibody or antibodies in the test sample are compared to the levels of anti-glycan antibodies in a control sample. The control sample is chosen from a group that includes one or more individuals known to have or not to have a gastrointestinal disorder, or to have or not to have a gastrointestinal disorder other than Crohn's disease. When the control sample is from an individual or individuals that do not have Crohn's disease, or has a gastrointestinal disease other than Crohn's disease, elevated levels in the test sample relative to the control sample indicates that the subject has Crohn's disease.

In some embodiments, the control sample is from one or more individuals with a gastrointestinal disorder that is irritable bowel syndrome or ulcerative colitis or other digestive diseases. In some embodiments, the control sample is from one or more individuals that do not have a gastrointestinal disorder.

In various embodiments, at least 2, 3, 4, 5, 6, or all of these antibodies are detected.

In some embodiments, the method further includes determining whether the test sample has an anti-Mannan antibody, which is also known as an anti-Saccharomyces cerevisiae antibody (ASCA). The presence of the anti-Mannan antibody in the sample indicates the subject has Crohn's Disease.

In some embodiments, the method further includes determining whether the test sample has an anti-neutrophil cytoplasmic antibodies (ANCA). The presence of ANCA indicates the subject does not have Crohn's Disease but may have Ulcerative Colitis.

The test sample can be, e.g., a biological fluid. Examples of biological fluids include, e.g., whole blood, serum, plasma, spinal cord fluid, urine, or saliva.

In some embodiments, one, two, three, or all four of an anti-Glc (β 1-3) Glc (β) antibody, anti-Man (α 1-3) Man (α) antibody, anti-Man (α 1-3)[Man (cc 1-6)] Man (α) antibodies, and/or anti-Mannan antibodies are detected.

The method can optionally include determining the isotype of the antibody. For example the method can include determining whether the antibody is an IgM, IgA, or IgG-type antibody. In some embodiments, the method is used to identify and compare an anti-Glc (β) IgG antibody, an anti-Glc (β 1-3) Glc (β) IgG antibody, an anti-Glc (β 1-4) Glc (β) IgG antibody, an anti-GlcNAc (β) 6-sulfate IgG antibody, an anti-Man (α) IgG antibody, an anti-Man (α 1-3) [Man (α1-6)] Man (β) IgG antibody, an anti-Man (α 1-3) Man (α) IgG antibody, an anti-Mannan IgG antibody an anti-Mannan IgA antibody, an anti-Xylan IgG antibody, or an anti-Man (α 1-2) Man

IgG antibody.

In some embodiments, a subject is scored as having CD if the test sample has elevated levels of IgG anti-Glc (β 1-3) Glc (β), or IgG anti anti-Man (α 1-3) Man (α), or IgG anti Mannan (ASCA) antibodies, or IgA anti Mannan (ASCA) antibodies, but does not have elevated levels of ANCA.

In some embodiments, a subject is scored as having IBD if the test sample has elevated levels of IgG anti-Glc (β 1-3) Glc (β), or IgG anti anti-Man (α 1-3) Man (α), or IgG anti Mannan (ASCA) antibodies, or IgA anti Mannan (ASCA) antibodies, or ANCA.

In some embodiments, the anti-glycan antibody or antibodies are detected using a fluorescent antibody, or are detected using an enzyme-linked immunoabsorbent assay (ELISA).

The test sample can be, e.g., a biological fluid. Examples of biological fluids include, e.g., whole blood, serum, plasma, spinal cord fluid, urine, or saliva.

The method can optionally include determining the isotype of the antibody. For example the method can include determining whether the antibody is an IgM, IgA, or IgG-type antibody.

Also within the invention are arrays that include reagents (preferably carbohydrate reagents) that specifically detect the CD-detecting antibodies disclosed herein, e.g., an anti-Glc (β) antibody, an anti-Glc (β 1-4) Glc (β) antibody, an anti-Glc (β 1-3) Glc (β) antibody, an anti-GlcNAc 6-sulfate (β) antibody, an anti-Man (α 1-2) Man (α) antibody, an anti-Man (α 1-3) Man (α) antibody, an anti-Man (α 1-6) Man (α) antibody, an anti-Man (α) antibody, an anti-Man (a 1-3)[Man (α 1-6)] Man (α), an anti-Mannan antibody, an anti-Dextran antibody, an anti-Xylan antibody, an anti-GlcNAc (β 1-4) GlcNAc (β) antibody, an anti-Gal 3 sulphate (β) antibody, an anti-aGlcNAc (β 1-3) GalNAc (β) antibody, an anti-GlcNAc (β 1-3) Gal β 1-4) Glc (β) antibody, or an anti-Gal (α 1-3) Gal (β1-4) GlcNAc (β) antibody for diagnosing CD.

In some embodiments, the reagents that are used to specifically bind and detect those anti glycans antibodies are the specific glycan structures. In other embodiments, the reagents are other molecules or macromolecules that include the specific glycan structure. For example, the anti-Glc (β 1-3) Glc (β) antibody can be detected using the polysaccharide β-D (1-3) Glucan, a polymer of glucose units connected in a (β, 1-3) glycosidic bond. Thus, the glycan itself can be used for detecting the corresponding antibody or antibodies, as can any carbohydrate, peptide, protein, or any other molecular structure that includes the glycan.

The array may additionally include a reagent or reagent, e.g., a carbohydrate reagent or reagents, that detect an anti-Mannan (ASCA) antibody or a ANCA.

In some embodiments, the glycans are attached to the array via a linker. A suitable linker includes at least one ethylene glycol derivative, at least two cyanuric chloride derivatives and an anilino group.

In some embodiment, at least two of the reagent or reagents are provided at the same location on the addressable array.

The invention additionally provides kits that include reagents for detecting anti-glycan antibodies that reveal the presence of Crohn's Disease. The kits includes one or more carbohydrate reagent(s) that specifically reacts with an anti-Glc (β) antibody, an anti-Glc (β 1-4) Glc (β) antibody, an anti-Glc (β 1-3) Glc (β) antibody, an anti-GlcNAc 6-sulfate (β) antibody, an anti-Man (α 1-2) Man (α) antibody, an anti-Man (α 1-3) Man (α) antibody, an anti-Man (α 1-6) Man (α) antibody, an anti-Man (α) antibody, an anti-Man (α 1-3)[Man (α 1-6)] Man (α), an anti-Mannan antibody, an anti-Dextran antibody, an anti-Xylan antibody, an anti-GlcNAc (β 1-4) GlcNAc (β) antibody, an anti-Gal 3 sulphate (β) antibody, an anti-aGlcNAc (β1-3) GalNAc (β) antibody, an anti-GlcNAc (β 1-3) Gal (β 1-4) Glc (β) antibody, and/or an anti-Gal (α 1-3) Gal (β 1-4) GlcNAc (β) antibody. In some embodiments, the kits contain directions for using the kits to perform the methods described herein. The kits may optionally include reagents for detecting antibody isotypes (e.g., IgA, IgG, and IgM antibodies).

In some embodiments, the kits include reagents that are used to specifically bind and detect those anti glycans antibodies that are the specific glycan structures. In other embodiments, the reagents in the kits are other molecules or macromolecules that include the specific glycan structure. For example, the anti-Glc (β 1-3) Glc (β) antibody can be detected using the polysaccharide β-D (1-3) Glucan, a polymer of glucose units connected in a (β, 1-3) glycosidic bond. Thus, the glycan itself can be used for detecting the corresponding antibody or antibodies, as can any carbohydrate, peptide, protein, or any other molecular structure that includes the glycan.

In some embodiments, the kits include reagents that are used to specifically bind and detect ASCA and/or ANCA.

Also within the invention is a method of diagnosing anti-phospholipid syndrome in a subject by providing a test sample from a subject and detecting in the test sample an anti-chitobiose antibody. Levels of the anti-GlcNAc (β 1-4) GlcNAc (β) antibody in the test sample are compared to the levels of the antibody in a control sample. The control sample is selected from group of one or more individuals known to have or not to have anti-phospholipid syndrome. When the control sample has one or more individuals that to not have APS, an elevated level of anti-GlcNAc (β 1-4) GlcNAc (β) antibodies in the test sample as compared to the control sample indicates the subject has APS.

In some embodiments, the method also includes detecting binding to a β-2 glycoprotein, and comparing the level of binding to the β-2 glycoprotein in the test sample to the level of binding to β-2 glycoprotein in the control sample. Increased binding to the β-2 glycoprotein in the test sample relative to a control sample taken from a non-APS individual or individuals indicates the subject has APS.

The test sample can be, e.g., a biological fluid. Examples of biological fluids include, e.g., whole blood, serum, plasma, spinal cord fluid, urine, or saliva.

The method can optionally include determining the isotype of the antibody. For example the method can include determining whether the antibody is an IgM, IgA, or IgG-type antibody. Also within the invention is an array that includes a reagent (preferably a carbohydrate reagent) that specifically detects and anti-GlcNAc (β 1-4) GlcNAc (β) antibody and (optionally) a reagent that detects a β-2 glycoprotein for detecting APS.

The invention additionally provides kits for diagnosing APS that include reagents for detecting an anti-chitobiose antibody and (optionally) a β-2 glycoprotein. In some embodiments, the kits contain directions for using the kits to perform the methods described herein.

Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by a person of ordinary skill in the art to which this invention belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, suitable methods and materials are described below. All publications, patent applications, patent, and other references mentioned herein are incorporated by reference in their entirety. In the case of conflict, the present specification, including definitions, will control. In addition, the materials, methods, and examples are illustrative only and not intended to be limiting.

Other features and advantages of the invention will be apparent from the following detailed description and claims.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 is a graph showing a Receiver Operator Characteristic (ROC) curve for differentiation between individuals with CD and individuals with other digestive diseases according to different anti glycans antibodies.

FIGS. 2A and 2B are histograms showing binding of anti-glycan antibodies to the indicated glycans in sera from patients with anti-phospholipid syndrome (APS).

DETAILED DESCRIPTION OF THE INVENTION

Crohn's disease (CD) and anti-phospholipid syndrome (APS) are diagnosed by examining a test sample from a subject for antibodies to one or more specific glycans. The presence of the antibodies in the test sample indicates the subject has CD or APS. In some embodiments, elevated levels of glycans in a test sample from the subject as compared to the levels of the glycan or glycans in a reference sample that does not have CD indicates that the subject has CD. The methods can be used distinguish the presence of CD in a subject from other inflammatory bowel diseases (including ulcerative colitis).

A translation of the LinearCode™ syntax used to describe glycan structure to IUPAC nomenclature can be found in Table 1. The glycans are presented either in the International Union of Pure and Applied Chemistry (IUPAC) condensed form for nomenclature carbohydrate representation or in LINEARCODE® syntax, for linear code syntax principles see (Banin E. Neuberger Y. Altshuler Y. Halevi A. Inbar O. Dotan N. and Dukler A. (2002) A Novel Linear Code Nomenclature for complex Carbohydrates. Trends in Glycoscience and Glycotechnology Vol. 14 No. 77 pp. 127-137). Translation of LINEARCODE® to IUPAC representation is in Table 1. All the glycan structures that discussed in this disclosure, unless mentioned otherwise are connected to in the indicated anomericity α or β to other molecular structure, linker, or solid phase.

As used herein, the term “inflammatory bowel disease” is synonymous with “IBD” and is a collective term referring to both Crohn's disease and ulcerative colitis. Thus, an individual having either Crohn's disease or ulcerative colitis is defined herein as having IBD. Conversely, an individual having neither ulcerative colitis nor Crohn's disease does not have IBD as defined herein. The term “inflammatory bowel disease” distinguishes Crohn's disease and ulcerative colitis from all other disorders, syndromes or abnormalities of the gastroenterological tract including irritable bowel syndrome.

The methods for diagnosing IBD involve determining whether a sample is positive for anti-neutrophil cytoplasmic antibodies (ANCA). Anti-neutrophil cytoplasmic antibodies that produce a perinuclear staining pattern (pANCA) are elevated in 60-80% of UC patients and less frequently in CD and other disorders of the colon. Serum titers of ANCA are elevated in UC patients regardless of clinical status and, thus, do not reflect disease activity. High levels of serum ANCA also persist in UC patients five years post-colectomy. Although pANCA is found only very rarely in healthy adults and children, healthy relatives of UC patients have an increased frequency of pANCA, indicating that pANCA may be an immunogenetic susceptibility marker. ANCA reactivity is also present in a small portion of patients with Crohn's disease. The reported prevalence in CD varies, with most studies reporting that 10 to 30% of CD patients express ANCA (Saxon et al., J. Allergy Clin. Immunol. 86:202-210 (1990); Cambridge et al., Gut 33:668-674 (1992); Pool et al., Gut 3446-50 (1993); and Brokroelofs et al., Dig. Dis. Sci. 39:545-549 (1994)).

As used herein, the term “anti-neutrophil cytoplasmic antibody” is synonymous with “ANCA” and means antibodies to cytoplasmic components of a neutrophil. ANCA, such as serum or saliva ANCA, can be detected using an enzyme-linked immunosorbent assay with alcohol-fixed neutrophils. As disclosed herein, ANCA activity is divided into several broad categories: perinuclear to nuclear staining or cytoplasmic staining with perinuclear highlighting (pANCA); cytoplasmic neutrophil staining without perinuclear highlighting (cANCA); and diffuse staining with speckling across the entire neutrophil (SAPPA). The term ANCA, as used herein, encompasses all varieties of anti-neutrophils cytoplasmic reactivity, including pANCA, cANCA and SAPPA. Similarly, the term “ANCA” encompasses all immunoglobulin isotypes including, for example, immunoglobulin A and G.

The determination of whether a sample is positive for ANCA using non-histological means is made using antigen specific for ANCA using methods described in U.S. Pat. No. 6,218,129. Such an antigen specific for ANCA can be, for example, whole fixed neutrophils; an unpurified or partially purified neutrophil extract; a purified UC pANCA antigen such as a purified protein, protein fragment or synthetically produced peptide; an anti-ANCA idiotypic antibody; or the like. Particularly useful antigens specific for ANCA are peptides, which can be chemically synthesized or expressed on the surface of phage. Purified antigens specific for ANCA can be, for example, histone H1, or an ANCA-reactive fragment of histone H1, as described in U.S. Pat. No. 6,074,835 now U.S. Pat. No. 6,074,835; an ulcerative colitis pANCA secretory vesicle antigen or an ANCA-reactive fragment thereof; or a microbial UC pANCA antigen, such as a histone H1-like antigen, porin antigen, Bacteroides antigen, or ANCA-reactive fragment thereof, as described in U.S. Pat. No. 6,033,864 now U.S. Pat. No. 6,033,864. One skilled in the art understands that additional antigens specific for ANCA, including antigenic fragments and ANCA-reactive peptides, can be identified, for example, using a representative UC pANCA monoclonal antibody.

Generating an Anti-Glycan Antibody Profile

In performing the methods of the invention, a sample to be analyzed is obtained from the subject to be diagnosed. The term “sample,” as used herein, means any biological specimen obtained from an individual that contains antibodies. A sample can be, for example, whole blood, plasma, saliva or other bodily fluid or tissue having antibodies, preferably a serum sample. Samples can diluted if desired before they are analyzed for anti-glycan antibodies. The subject can be, e.g., a human, a non-human primate (including a chimpanzee, ape, gorilla, old world primate), cow, horse, dog, cat, pig, goat, sheep, rodent (including, e.g., a mouse, rat, or guinea pig) Anti-glycan profiles can be determined by using methods known in the art for identifying antibodies to glycans. The methods include those disclosed in e.g., WO00/49412, or WO02/064556, or Schwarz et al., Glycobiology 13:749-54, 2003.

The methods are typically performed using reagents that specifically bind to the anti-glycan antibodies. The reagents can be, e.g., the specific glycan structures. Alternatively, the reagents can be other molecules or macromolecules that include the specific glycan structure. For example, the anti-Glc (β 1-3) Glc (β) antibody can be detected using the polysaccharide β-D (1-3) Glucan, a polymer of glucose units connected in a (β 1-3) glycosidic bond. Thus, the glycan itself can be used for detecting the corresponding antibody or antibodies, as can any carbohydrate, peptide, protein, or any other molecular structure that includes the glycan.

Glycan antigens used to identify various anti-glycan antibodies can be obtained from a variety of other sources so long as the antigen is capable of binding specifically to the given anti-glycan Binding to anti-glycan antibodies can be performed using variety of other immunoassay formats known in the art, including competitive and non-competitive immunoassay formats can also be used (Self and Cook, Curr. Opin. Biotechnol. 7:60-65 (1996), which is incorporated by reference). Other assays include immunoassays, such as enzyme-linked immunosorbent assays (ELISAs). An enzyme such as horseradish peroxidase (HRP), alkaline phosphatase (AP), β-galactosidase or urease can be linked to a secondary antibody selective for a primary anti-glycan antibody of interest. A horseradish-peroxidase detection system can be used, for example, with the chromogenic substrate tetramethylbenzidine (TMB), which yields a soluble product in the presence of hydrogen peroxide that is detectable at 450 nm. An alkaline phosphatase detection system can be used with the chromogenic substrate p-nitrophenyl phosphate, for example, which yields a soluble product readily detectable at 405 nm. Similarly, a β-galactosidase detection system can be used with the chromogenic substrate o-nitrophenyl- a β-D-galactopyranoside (ONPG), which yields a soluble product detectable at 410 nm, or a urease detection system can be used with a substrate such as urea-bromocresol purple (Sigma Immunochemicals, St. Louis, Mo.). A useful secondary antibody linked to an enzyme can be obtained from a number of commercial sources; goat F(ab′)₂ anti-human IgG-alkaline phosphatase, for example, can be purchased from Jackson Immuno-Research (West Grove, Pa.).

Immunoassays encompass capillary electrophoresis based immunoassays (CEIA) and can be automated, if desired. Immunoassays also can be used in conjunction with laser induced fluorescence (see, for example, Schmalzing and Nashabeh, Electrophoresis 18:2184-93 (1997)); Bao, J. Chromatogr. B. Biomed. Sci. 699:463-80 (1997), each of which is incorporated herein by reference). Liposome immunoassays, such as flow-injection liposome immunoassays and liposome immunosensors, also can be used (Rongen et al., J. Immunol. Methods 204:105-133 (1997)).

A radioimmunoassay can also be used for determining whether a sample is positive for a glycan antibody, or for determining the level of anti-glycan antibodies in a sample. A radioimmunoassay using, for example, an ¹²⁵iodine-labeled secondary antibody (Harlow and Lane, Antibodies A Laboratory Manual Cold Spring Harbor Laboratory: New York, 1988, which is incorporated herein by reference) is encompassed within the invention.

A secondary antibody may alternatively be labeled with a chemiluminescent marker. Such a chemiluminescent secondary antibody is convenient for sensitive, non-radioactive detection of anti-glycan antibodies and can be obtained commercially from various sources such as Amersham Lifesciences, Inc. (Arlington Heights, Ill.).

A detectable reagent may also be labeled with a fluorochrome. Appropriate fluorochromes include, for example, DAPI, fluorescein, Hoechst. 33258, R-phycocyanin, B-phycoerythrin, R-phycoerythrin, rhodamine, Texas red or lissamine. A particularly useful fluorochrome is fluorescein or rhodamine. Secondary antibodies linked to fluorochromes can be obtained commercially. For example, goat F(ab′)₂ anti-human IgG-FITC is available from Tago Immunologicals (Burlingame, Calif.).

A signal from the detectable reagent can be analyzed, for example, using a spectrophotometer to detect color from a chromogenic substrate; a radiation counter to detect radiation, such as a gamma counter for detection of ¹²⁵ Iodine; or a fluorometer to detect fluorescence in the presence of light of a certain wavelength. For detection of enzyme-linked reagents, a quantitative analysis of the amount of anti-glycan antibodies can be made using a spectrophotometer such as an EMAX Microplate Reader (Molecular Devices, Menlo Park, Calif.) in accordance with the manufacturer's instructions. If desired, the assays of the invention can be automated or performed robotically, and the signal from multiple samples can be detected simultaneously.

Other methods include, e.g., flow cytometry (including bead based immunoassays), and phage display technology for expressing a recombinant antigen specific for an anti-glycan antibody. Phage particles expressing the antigen specific for a desired anti-glycan antibody can be anchored, if desired, to a multiwell plate using an antibody such as an anti phage monoclonal antibody (Felici et al., “Phage-Displayed Peptides as Tools for Characterization of Human Sera” in Abelson (Ed.), Methods in Enzymol. 267, San Diego: Academic Press, Inc. (1996), which is incorporated by reference herein).

Anti-glycan antibodies are conveniently performed by simultaneously analyzing multiple samples for CD for the presence of one or more anti-glycan antibodies. For example, the antibodies can be detected using an array of reagents that can bind specifically to the anti glycan antibodies. Preferably, each reagent is provided in a different location with a defined address on the array. By exposing the sample to array all the anti glycan antibodies that bind to the reagent on the array can be detected in one test Suitable arrays that include reagents (preferably carbohydrate reagents) that specifically detect the CD-detecting antibodies disclosed herein, e.g., an anti-Glc (β) antibody, an anti-Glc (β 1-4) Glc (β) antibody, an anti-Glc (β 1-3) Glc (β) antibody, an anti-GlcNAc 6-sulfate (β) antibody, an anti-Man (α 1-2) Man (α) antibody, an anti-Man (α 1-3) Man (α) antibody, an anti-Man (α 1-6) Man (α) antibody, an anti-Man (α) antibody, an anti-Man (α 1-3)[Man (α 1-6)] Man (α), an anti-Manna antibody, an anti-Dextran antibody, an anti-Xylan antibody, an anti-GlcNAc (β 1-4) GlcNAc (β) antibody, an anti-Gal 3 sulphate (β) antibody, an anti-aGlcNAc (β 1-3) GalNAc (β) antibody, an anti-GlcNAc (β 1-3) Gal (β 1-4) Glc (β) antibody, or an anti-Gal (α 1-3) Gal (β 1-4) GlcNAc (β) antibody for diagnosing CD.

In some embodiments, the reagents that are used to specifically bind and detect those anti glycans antibodies are the specific glycan structures. In other embodiments, the reagents are other molecules or macromolecules that include the specific glycan structure. For example, the anti-Glc (β 1-3) Glc (β) antibody can be detected using the polysaccharide β-D (1-3) Glucan, a polymer of glucose units connected in a (β, 1-3) glycosidic bond. Thus, the glycan itself can be used for detecting the corresponding antibody or antibodies, as can any carbohydrate, peptide, protein, or any other molecular structure that includes the glycan.

The array may additionally include a reagent or reagent, e.g., a carbohydrate reagent or reagents, that detect an anti-Mannan antibodies or a ANCA. In some embodiments, the glycans are attached to the array via a linker. A suitable linker includes at least one ethylene glycol derivative, at least two cyanuric chloride derivatives and an anilino group.

Arrays useful for diagnosing APD can include a reagent (preferably a carbohydrate reagent) that specifically detects an anti-chitobiose antibody and, optionally, a reagent that specifically detects a β-2 glycoprotein for detecting.

Interpreting Anti-Glycan Antibody Binding Data

Typically, binding of anti-glycan antibodies to glycans in a sample is compared to a reference population, and differences in levels of the anti-glycan antibodies in the two samples are compared. The threshold for determining whether a test sample is scored positive for CD or APS based on its ant-glycan antibody profile can be altered depending on the sensitivity or specificity desired. The clinical parameters of sensitivity, specificity, negative predictive value, positive predictive value and overall agreement are calculated using true positives, false positives, false negatives and true negatives. A “true positive” sample is a sample positive for CD according to colonoscopy, radiologic and/or histologic analysis, which is also diagnosed positive according to a method of the invention. A “false positive” sample is a sample negative for CD by colonoscopic, radiologic and/or histologic analysis, which is diagnosed positive according to a method of the invention. Similarly, a “false negative” is a sample positive for CD by colonoscopic, radiologic and/or histologic analysis, which is diagnosed negative according to a method of the invention. A “true negative” is a sample negative for CD by colonoscopic, radiologic and/or histologic analysis, and also negative for CD according to a method of the invention. See, for example, Mousy (Ed.), Intuitive Biostatistics New York: Oxford University Press (1995), which is incorporated herein by reference.

As used herein, the term “sensitivity” means the probability that a laboratory method is positive in the presence of CD. Sensitivity is calculated as the number of true positive results divided by the sum of the true positives and false negatives. Sensitivity essentially is a measure of how well a method correctly identifies those with disease. In a method of the invention, the anti-glycan antibody values can be selected such that the sensitivity of diagnosing an individual is at least about 60%, and can be, for example, at least about 65%, 70%, 75%, 80%, 85%, 90% or 95%.

As used herein, the term “specificity” means the probability that a method is negative in the absence of CD. Specificity is calculated as the number of true negative results divided by the sum of the true negatives and false positives. Specificity essentially is a measure of how well a method excludes those who do not have CD. The anti-glycan cut-off value can be selected such that, when the sensitivity is at least about 70%, the specificity of diagnosing an individual is in the range of 30-60%, for example, 35-60%, 40-60%, 45-60% or 50-60%.

The term “positive predictive value,” as used herein, is synonymous with “PPV” and means the probability that an individual diagnosed as having CD actually has the disease. Positive predictive value can be calculated as the number of true positives divided by the sum of the true positives and false positives. Positive predictive value is determined by the characteristics of the diagnostic method as well as the prevalence of the disease in the population analyzed. In a method of the invention, the anti-glycan antibody cut-off values can be selected such that the positive predictive value of the method in a population having a CD disease prevalence of 15% is at least about 5%, and can be, for example, at least about 8%, 10%, 15%, 20%, 25%, 30% or 40%.

As used herein, the term “overall agreement” means the accuracy with which a method diagnoses a disease state. Overall agreement is calculated as the sum of the true positives and true negatives divided by the total number of sample results and is affected by the prevalence of CD in the population analyzed. The anti-glycan antibody cut-off values can be selected such that the overall agreement of a method of the invention in a patient population having an CD disease prevalence of 15% is at least about 45%, and can be, for example, at least about 50%, 55% or 60%.

The invention will be illustrated in the following non-limiting examples.

Example 1 Comparative Antiglycan Antibody Levels in the Serum of Crohn's Disease Patients and Patients with Other Digestive Diseases

An anti-glycan antibody profile for IgG, IgA and IgM in the serum of the patients was obtained using GlycoChip® arrays (Glycominds, Ltd., Lod, Israel, Cat No. 9100). The arrays were constructed using procedures described in Schwarz et. al. Glycobiology vol. 13 no. 11 pp. 749-754, 2003. Anti-glycan antibody profiles of 45 CD patients and 27 patients with other digestive diseases were compared.

All serum samples were tested using GlycoChip® plates (Glycominds Ltd., Lod, Israel, Cat No. 9100), which was an array of mono and oligosaccharides covalently attached to a reduced volume 384 wells micro titer plate. The mono and oligosaccharides displayed on the array are listed in Table 1. A translation of the LinearCode™ syntax used to describe glycan structure to IUPAC nomenclature can be found in Table 1.

The sera from patients volunteers who had signed an informed consent form were collected by Dr. Iris Dotan for the Gastroenterology and liver disease institute in the Tel Aviv Sorasky Medical center, Israel. All patient were diagnosed by Dr. Iris Dotan. The sera were collected in evacuated silicon coated gel containing tubes (Estar Technologies Cat# 616603GLV). The sera were separated from the blood cells and kept frozen in −25° C. until use. The volume of all solutions added to the glycan array was 10 μl/well. The sera were diluted (1:20; saturating concentration) in 0.15M Tris-HCl pH 7.2, 0.085M Mg2SO4, 0.05% Tween 20 (TBST) containing 1% BSA (Sigma), dispensed into glycan array plates using a Tecan Genesis Workstation 200 automated handling system, and incubated for 60 min at 37° C. The plates were then washed with 250 μL/well Phosphate buffered Saline with 0.05% Tween 20 (PBST, Sigma) in an automatic plate washer (Tecan, POWERWASHER™). At this point the following reagents, diluted in TBST with 1% BSA, were added using a Multidrop 384 dispenser (Thermo Labsystems) and incubated for 60 min at 37° C.: for IgG, IgA, and IgM determination—the respective sub-class specific biotinylated goat anti-human Ig antibody (Jackson, Pa., USA) at 2.8 μg/ml, 3 μg/ml, and 0.9 μg/ml, respectively. Following washing with PBST, Streptavidin-conjugated europium (0.1 μg/ml) diluted in TBST with 1% BSA was added to each well followed by incubation for 30 min at 37° C. in the dark, and washing with PBST. DELFIA™ enhancement solution was then added to the wells and the plates were incubated for 30 to 45 min in the dark at room temperature. The fluorescence of the wells was read with a Victor 1420 (Wallac, Finland) plate reader using time resolved fluorescence settings of 340/612 nm (Excitation/Emission). Some patients were tested for the presence of antibodies to perinuclear anti neutrophil cytoplasmic antibodies (pANCA) and anti-Saccharomyces cerevisiae (ASCA) IgG and IgA using a commercial kits made by INOVA, San-Diego Calif. cat. No 708290, 708865, 708870 respectively according to the manufacturer instructions.

Tables 2 and 3 represent the levels of IgG, IgA and IgM type anti bodies anti glycan antibodies that were detected at significantly different levels between the CD population and the population with other digestive diseases. The values presented are absolute values after reduction of background. The back ground signal was measured as the signal received from wells with covalently bound p-nithrophenol. If the result was negative the signal was scored as zero.

Comparison of the average and median values of anti-carbohydrate antibodies in the CD and other digestive disease populations reveals a significant elevation in most of the anti glycans antibodies in the CD group as compared to the group containing individuals with the other digestive diseases group. No one of the CD patients was found to be positive for pANCA antibodies. All the anti glycans levels that are displayed in Tables 2 and 3 show statistically significant (α=0.05; p<0.05) different between the CD groups and the other digestive disease or normal group. Statistically significant differences between the medians of signals of CD and other digestive disease population and normal population were observed for antibodies bound to the following glycans: Glc (β), Glc (β 1-4) Glc (β), Glc (β 1-3) Glc (β), GlcNAc 6-sulfate (β), Man (α 1-2) Man (α), Man (α 1-3) Man (α), Man (α 1-6) Man (α), Man (α), Man (α 1-3)[Man (α 1-6)] Man (α), Mannan, Dextran, Xylan, GlcNAc (β 1-4) GlcNAc (β), Gal 3 sulphate (β), GlcNAc (β1-3) GalNAc (β), GlcNAc (β 1-3) Gal (β 1-4) Glc (β), and Gal (α 1-3) Gal (β1-4) GlcNAc (β).

Table 4 describes the specificity and sensitivity of the different IgG anti glycans for differentiation between CD and other digestive diseases using different cut-off values. The cutoff values for each glycans where set as the 89^(th) percentile of the non CD group.

These results reveal a set of chemically defined glycan antigens that are useful for diagnosing CD. The levels of antibodies to those glycans are higher in the CD population than in the population of normal individuals or individuals with other digestive diseases. The antibodies that showed the greatest differentiation between CD and other digestive diseases in these studies are a set of antibodies to mannose based glycan fragment as well as antibodies to Glc (β), Glc (β 1-4) Glc (β), Glc (β1-3) Glc (β). Antibodies to Glc (β 1-3) Glc (β), Man (α 1-3) Man (α) and Man (α 1-3) [Man (α 1-6)] Man (α) were in particular able to differentiate between CD and other digestive disease at 57-62% sensitivity and 89%-93% specificity. The separation of those structures was better than what was achieved with Mannan (ASCA) 47% sensitivity and 89% specificity. Table 4 demonstrates that it is possible to use different cut-off levels and to achieve higher sensitivity but lower specificity. Table 5 describes the sensitivity, specificity, True Positives (TP), True Negative (TN), False Positives (FP), and False Negatives (FN) and positive Predictive value (PPV) in different cut-off value for differentiation between CD and other digestive disease according to the level of Anti Glc (β 1-3) Glc (β), IgG and anti Mannan IgG. FIG. 1 is a Receiver Operator Characteristic (ROC) curve for differentiation between individuals with CD and individuals with other digestive diseases according to levels of anti Glc (B), IgG and anti Mannan IgG antibodies

By using combination of two or more glycans it is possible to improve the sensitivity without reducing the specificity. For example by setting cut-offs of 2000,000 for anti Glc (β 1-3) Glc (β) and 2,400,000 for anti Mannan and setting the criteria for identification of CD as individuals who are above cut-off in either of the antibodies it is possible to achieve 82% sensitivity with 70% specificity. Achieving this sensitivity by each of the antibodies alone would require to set lower cut-offs that would lead to poor specificity (37% for Glc (β1-3) Glc (β)).

Example 2 Levels of Antiglycan Antibody Levels in the Serum of Anti-Phospholipid Syndrome (APS) Patients and Patients with Other Digestive Diseases

A pool of serum samples from APS patients was fractionated on a 13-2 glycoprotein column and tested for the presence of anti-glycan antibodies

Antibody binding was examined using GLYCOCHIP™ substrates as described in WO00/49412. Wells were blocked with ddH₂O/BSA 2.5%. The serum sample was diluted 1:2 in 1% TBST/BSA. Anti-IgA, IgG, and IgM samples were diluted 1:100 in TBST/BSA 1%. The Alexa 633 dye (Molecular Probes, Eugene, Oreg., # S-20992) was diluted 1:150 in TBST 1:150. Samples were injected using a Tescan HS4800 program. Dry arrays were scanned using an Affymetrix 428 Scanner, and images were analyzed using ‘ArrayPro’ software. Numerical values were exported to Excel and analyzed. For isotype determination, anti-human IgG, Fc gamma fragment specific\Biotin (Goat); Jackson; Cat # 109-065-008, anti-human IgM, Fc 5 mu fragment specific\Biotin (Goat); Jackson; Cat # 109-065-043, and anti-human serum IgA\biotin (Goat); Jackson; Cat # 109-065-011.

The serum sample was affinity-purified against a column of β-2-glycoprotein and then applied to a GlycoChip containing multiple glycans. The full magnitude (0-2.5×10⁷) of the interaction profile of the APS immunoglobulins and the tested glycans antibodies on the GlycoChip are shown in FIG. 2A. A smaller scale (0-5×10⁶) of the binding is shown in FIG. 2B.

The highest levels of antibodies were observed for antibodies against GlcNAc (β 1-4) GlcNAc (β) for each of the IgA, IgG, and IGM subclasses High IgG levels against LPS from Salmonella typhimurium, Man (α 1-6) Man (α), GlcNAc (α), GlcNAc (β) and Gal (β 1-4) Glc (β) (Lactose) were also observed.

IgA levels were highest against GlcNAc (β 1-4) GlcNAc (β) and relatively high against Gal (β 1-4) Glc (β) and LPS from Salmonella. IgM levels were highest against GlcNAc (β 1-4) GlcNAc (β) and relatively high against LPS from E. coli O26:B6, Glucose derivatives such as Glc (α), Glc (α 1-4) Glc (α), Glc (α 1-4) Glc (β), Glc (β 1-3) Glc (β) and Glc (β 1-4) Glc (β), GlcNAc (α), Rha (α) as well as Man (α 1-6) Man (α), GalA (β), GlcA (β) and LPS from Salmonella. Relatively low levels of Ig were detected against the preparation of Mannan used on the GlycoChip substrate.

These results demonstrate that elevated levels antibodies to GlcNAc (β 1-4) GlcNAc (β) in the blood may serve as a marker for diagnosis of APS, and/or for the severity of the disease.

Other Embodiments

It is to be understood that while the invention has been described in conjunction with the detailed description thereof, the foregoing description is intended to illustrate and not limit the scope of the invention, which is defined by the scope of the appended claims. Other aspects, advantages, and modifications are within the scope of the following claims.

TABLE 1 Saccharides displayed on the glycan array Glycan IUPAC LINEARCODE ® Common Name 0 p-Nitronhenol pNP-0 1 Gal (α) Aa 2 Gal (β) Ab 3 Gal (β 1-3) GalNAc (α) Ab3ANa 4 Gal (β 1-3) GlcNAc (β) Ab3GNb 5 Gal (β 1-4) Glc (β) Ab4Gb Lactose 6 Gal (β 1-6) Gal (β) Ab6Ab 7 GalNAc (α) ANa 8 GalNAc (β) ANb 9 Fuc (α) Fa 10 Fuc (β) Fb 11 Glc (α) Ga 12 Glc (α 1-4) Glc (α) Ga4Ga Maltose 13 Glc (α 1-4) Glc (β) Ga4Gb 14 Glc (β) Gb 15 Glc (β 1-4) Glc (β) Gb4Gb Cellobiose 16 Glc (β 1-4) Glc (β 1-4) Glc (β) Gb4Gb4Gb Cellotriose 17 GlcNAc (α) GNa 18 GlcNAc (β) GNb 19 GlcNAc (β 1-3) GalNAc (α) GNb3ANa 20 GlcNAc (β 1-4) GlcNAc (β) GNb4GNb Chitobiose 21 L-Rha (α) Ha 22 GalA (β) Lb 23 Man (α) Ma 24 Man (β) Mb 25 Neu5Ac (α) NNa 26 L-Araf (α) Ra 27 GlcA (β) Ub 28 X (α) Xa 29 X (β) Xb 30 Gal (β1-3) [GlcNAc (β1-6)] GalNAc (α) Ab3(GNb6)ANa 31 Gal (β 1-4) GlcNAc (α) Ab4GNa 32 Gal (α1-3) Gal (β 1-4) GlcNAc (β) Aa3Ab4GNb Linear B-2 33 Gal (β1-3) Gal (β1-4)GalNAc (β) Ab4GNb 34 Man (β1-4) GlcNAc (β) Mb4Gb 35 GlcNAc (β1-6)GalNAc (α) GNb6ANa 36 Fuc (α 1-2) Gal (β) Fa2Ab 37 Man (α 1-3) Man (α) Ma3Ma 38 GlcNAc (β) 6-sulfate GN[6S]b 39 Glc (β 1-3) Glc (β) Gb3Gb 40 Gal (β) 3-sulfate A[3S]b 41 Man (α 1-3) [Man (α1-6)] Man (β) Ma3(Ma6)Mb 42 GlcNAc (β 1-3) Gal (α1-4) Glc (β) GNb3Ab4Gb Lacto-3 43 Gal (α1-4) Gal (β1-4) Glc (β) Aa4Ab4Gb Pk antigen 44 Man (α 1-6) Man α Ma6Ma 45 Man (α 1-2) Man α Ma2Ma 46 Dextran Dextran 47 Mannam Mannam 48 Xylan Xylan

TABLE 2 Fluorescent signals from binding of antibodies to different glycans in CD patients and non CD patients. Glycans are presented in LINEARCODE ®. Patient No. Clinical condition IgG Gb IgG Gb3Gb IgG Gb4Gb IgG GN[6S]b 10,001 Crohn's disease 239,525 979,509 324,062 28,737 10,004 Crohn's disease 381,122 3,511,076 2,381,922 0 10,005 Crohn's disease 7,641 525,203 13,726 351,139 10,006 Crohn's disease 555,734 62,662 269,512 78,564 10,007 Crohn's disease 0 0 0 0 10,008 Crohn's disease 0 3,186,273 261,521 0 10,009 Crohn's disease 596,715 454,736 328,022 167,316 10,011 Crohn's disease 435,226 2,467,429 0 66,968 10,012 Crohn's disease 0 27,402 214,518 0 10,013 Crohn's disease 91,743 27,235 51,842 0 10,015 Crohn's disease 1,435,731 1,817,947 2,540,714 303,550 10,016 Crohn's disease 906,573 1,994,899 1,971,018 374,644 10,018 Crohn's disease 521,021 0 147,106 0 10,021 Crohn's disease 554,385 1,317,083 1,000,896 42,976 10,025 Crohn's disease 885,575 6,891,638 1,217,531 364,637 10,026 Crohn's disease 539,816 2,154,175 961,447 327,688 10,027 Crohn's disease 113,583 3,559,430 1,182,752 66,965 10,028 Crohn's disease 0 1,825,768 169,320 128,581 10,031 Crohn's disease 20,260 371,507 159,225 0 10,033 Crohn's disease 725,599 344,750 390,943 340,134 10,034 Crohn's disease 227,314 87,267 850,765 0 10,036 Crohn's disease 391,063 1,425,185 221,359 185,257 10,041 Crohn's disease 2,447,847 7,299,442 1,196,749 554,440 10,042 Crohn's disease 3,075,165 4,137,076 4,847,786 1,141,538 10,043 Crohn's disease 228,780 5,724,798 5,015,655 0 10,047 Crohn's disease 899,158 5,545,432 762,145 348,988 10,058 Crohn's disease 1,111,889 3,096,078 998,236 259,305 10,060 Crohn's disease 3,488,594 5,107,549 3,189,052 861,636 10,061 Crohn's disease 302,526 6,708,583 403,578 63,045 10,062 Crohn's disease 1,970,248 1,680,984 1,712,647 592,484 10,064 Crohn's disease 923,883 1,722,429 2,323,960 458,720 10,067 Crohn's disease 679,589 2,850,072 1,376,044 — 10,068 Crohn's disease 412,474 241,622 307,409 256,696 10,071 Crohn's disease 1,182,752 3,578,889 708,938 0 10,073 Crohn's disease 547,155 2,551,776 1,018,813 293,958 10,074 Crohn's disease 1,849,657 1,705,604 196,196 150,342 10,075 Crohn's disease 4,173,024 7,209,245 3,339,828 80,395 10,077 Crohn's disease 678,576 1,376,957 658,444 675,875 10,078 Crohn's disease 852,494 2,324,221 2,518,629 740,309 10,081 Crohn's disease 1,856,749 1,869,774 1,496,600 365,947 10,089 Crohn's disease 3,084,860 1,560,904 4,839,888 210,753 10,090 Crohn's disease 759,279 5,640,050 5,833,141 215,327 10,094 Crohn's disease 2,153,522 2,067,775 2,215,576 894,280 10,095 Crohn's disease 4,905,595 4,327,530 3,460,322 573,036 10,102 Crohn's disease 445,821 378,722 797,078 161,173 10,051 No digestive disease 549,212 526,443 196,870 947,346 10,052 No digestive disease 560,759 317,476 367,236 33,889 10,053 No digestive disease 57,858 242,565 0 50,803 10,054 No digestive disease 107,351 78,554 408,109 95,010 10,059 Anal fissure 349,679 441,239 0 0 10,066 Proctitis/Psoriasis 149,491 174,695 197,496 99,518 10,080 No digestive disease 948,567 1,266,954 885,097 67,661 10,082 No digestive disease 78,069 1,379,223 710,595 — 10,003 Ulcerative colitis 363,362 312,940 168,366 347,208 10,020 Ulcerative colitis — 2,703,085 — — 10,022 Ulcerative colitis 340,259 1,732,725 2,209,456 67,238 10,023 Ulcerative colitis 90,914 1,209,892 20,926 0 10,024 Ulcerative colitis 159,550 489,733 0 0 10,030 Ulcerative colitis 1,650 6,963 58,288 0 10,039 Ulcerative colitis 0 546,432 2,775,860 0 10,040 Ulcerative colitis 0 371,648 33,848 0 10,044 Ulcerative colitis 543,352 108,535 551,977 0 10,050 Ulcerative colitis 1,182,838 430,129 605,578 274,541 10,065 Ulcerative colitis 977,372 851,962 931,083 304,212 10,069 Ulcerative colitis 169,349 133,683 746,781 97,734 10,072 Ulcerative colitis 120,534 86,949 188,860 0 10,079 Ulcerative colitis 335,039 3,441,578 1,186,596 191,939 10,084 Ulcerative colitis 446,430 1,574,353 2,292,321 136,761 10,086 Ulcerative colitis 161,202 379,003 417,320 36,600 10,087 Ulcerative colitis 343,325 156,547 306,446 114,600 10,096 Ulcerative colitis 413,406 1,461,919 550,499 94,137 10,097 Ulcerative colitis 125,661 612,367 622,629 46,173 Cutoffs in percintiles 65 357,204 582,696 614,956 96,508 75 445,973 1,224,157 759,355 120,140 85 548,919 1,457,784 928,783 190,400 90 677,098 1,621,865 1,493,453 283,442 98 1,071,886 3,042,838 2,514,749 623,271 Avarage Crohn's disease 1,036,850 2,487,482 1,419,442 260,564 No Chrohn's Disease 317,600 779,174 608,638 111,310 Median Crohn's disease 596,715 1,880,984 961,447 185,257 No Chrohn's Disease 169,349 441,239 408,109 50,803 ttest CD vs Non CD 0.002456446 0.000184349 0.01128244 0.016974961 Patient No. Clinical condition IgG Ma IgG Ma3(Ma6)Mb IgG Ma3Ma IgG Mannan 10,001 Crohn's disease 412,186 145,841 585,222 5,407,769 10,004 Crohn's disease 0 0 1,014,289 3,676,579 10,005 Crohn's disease 0 0 0 3,732,911 10,006 Crohn's disease 42,543 0 48,213 1,091,472 10,007 Crohn's disease 0 0 0 2,448,511 10,008 Crohn's disease 0 0 0 6,261,035 10,009 Crohn's disease 0 46,886 128,896 3,208,701 10,011 Crohn's disease 0 0 0 4,269,038 10,012 Crohn's disease 0 0 0 372,869 10,013 Crohn's disease 83,080 0 0 592,410 10,015 Crohn's disease 0 0 257,538 6,989,275 10,016 Crohn's disease 84,710 30,478 0 7,307,560 10,018 Crohn's disease 0 0 0 609,882 10,021 Crohn's disease 0 0 0 3,891,841 10,025 Crohn's disease 188,355 109,598 452,964 7,130,311 10,026 Crohn's disease 153,606 123,522 257,506 3,708,910 10,027 Crohn's disease 0 84,390 224,520 4,915,018 10,028 Crohn's disease 0 0 0 602,554 10,031 Crohn's disease 57,754 0 328,085 5,299,212 10,033 Crohn's disease 19,046 0 34,499 8,102,888 10,034 Crohn's disease 9,877 0 35,552 4,729,753 10,036 Crohn's disease 479,128 155,385 279,542 4,912,482 10,041 Crohn's disease 1,382,815 1,131,592 1,065,763 5,218,211 10,042 Crohn's disease 657,599 790,941 1,710,324 2,611,948 10,043 Crohn's disease 0 0 1,436,359 4,450,715 10,047 Crohn's disease 11,887 — 15,398 3,536,621 10,058 Crohn's disease 284,314 365,078 320,510 — 10,060 Crohn's disease 66,108 886,832 — 4,557,274 10,061 Crohn's disease 116,816 84,505 12,868 737,419 10,062 Crohn's disease 479,672 91,350 287,315 5,190,198 10,064 Crohn's disease 1,300,332 2,380,047 1,161,372 6,597,714 10,067 Crohn's disease 325,777 929,191 583,323 1,998,817 10,068 Crohn's disease 501,122 1,525,473 961,710 29,236 10,071 Crohn's disease 309,375 289,185 1,189,325 3,463,114 10,073 Crohn's disease 1,159,788 1,611,290 1,181,541 6,630,916 10,074 Crohn's disease 1,020,256 1,307,139 952,214 4,060,050 10,075 Crohn's disease 1,048,834 749,060 2,574,514 6,247,571 10,077 Crohn's disease 420,437 209,185 586,814 228,636 10,078 Crohn's disease 794,975 676,514 725,854 6,306,500 10,081 Crohn's disease 851,804 125,827 824,214 3,758,249 10,089 Crohn's disease 483,336 0 502,368 179,822 10,090 Crohn's disease 908,572 122,830 250,375 6,317,456 10,094 Crohn's disease 1,237,060 930,156 491,528 4,098,131 10,095 Crohn's disease 797,142 379,977 393,270 6,473,836 10,102 Crohn's disease 245,130 126,639 88,512 115,577 10,051 No digestive disease 0 0 191,673 1,794,907 10,052 No digestive disease 5,587 0 58,482 356,353 10,053 No digestive disease 0 0 37,486 503,405 10,054 No digestive disease 0 0 0 694,824 10,059 Anal fissure 0 0 0 2,354,312 10,066 Proctitis/Psoriasis 117,444 0 74,490 1,088,575 10,080 No digestive disease — — — 1,159,558 10,082 No digestive disease — — — 4,238,190 10,003 Ulcerative colitis 50,843 — — 525,545 10,020 Ulcerative colitis — — — 1,186,666 10,022 Ulcerative colitis 0 0 0 4,272,493 10,023 Ulcerative colitis 267,614 0 0 308,161 10,024 Ulcerative colitis 0 0 0 5,003,204 10,030 Ulcerative colitis 71,007 0 0 1,227,240 10,039 Ulcerative colitis 0 0 0 1,588,845 10,040 Ulcerative colitis 0 0 0 2,838,390 10,044 Ulcerative colitis 274,235 0 19,948 558,985 10,050 Ulcerative colitis 419,442 118,678 313,631 1,537,328 10,065 Ulcerative colitis 128,015 133,662 314,068 1,911,575 10,069 Ulcerative colitis 0 0 0 1,281,592 10,072 Ulcerative colitis 69,365 6,299 120,392 3,436,295 10,079 Ulcerative colitis 103,676 48,476 34,897 1,513,149 10,084 Ulcerative colitis 184,468 22,962 21,668 1,096,196 10,086 Ulcerative colitis 0 0 0 1,699,125 10,087 Ulcerative colitis 137,647 57,993 240,234 1,047,544 10,096 Ulcerative colitis 54,108 — 34,517 4,957,039 10,097 Ulcerative colitis — — — 1,426,371 Cutoffs in percintiles 65 70,268 — 34,771 1,649,499 75 120,086 1,575 62,484 2,022,259 85 182,126 47,200 118,798 3,406,400 90 251,875 76,199 206,241 4,248,481 98 341,030 129,870 313,832 4,978,274 Avarage Crohn's disease 354,076 342,420 465,829 3,823,755 No Chrohn's Disease 69,757 14,373 54,133 1,837,254 Median Crohn's disease 153,606 91,350 279,542 4,060,050 No Chrohn's Disease — — — 1,426,371 ttest CD vs Non CD 0.0011162 0.002612017 0.00032205 0.00017892 IgG IgA Patient ASCA ASCA No. Clinical condition IgG Xylan IgG Ma2Ma (

pANCA (

10,001 Crohn's disease 4,677,868 0 10,004 Crohn's disease 2,190,867 956,611 10,005 Crohn's disease 3,488,820 0 10,006 Crohn's disease 324,492 276,481 10,007 Crohn's disease 1,002,289 0 10,008 Crohn's disease 2,793,801 169,975 80 neg 33 10,009 Crohn's disease 874,256 1,180,180 10,011 Crohn's disease 918,204 0 10,012 Crohn's disease 0 3,692 10,013 Crohn's disease 263,911 30,891 10,015 Crohn's disease 2,571,728 416,510 10,016 Crohn's disease 2,000,621 434,306 10,018 Crohn's disease 9,322 285,948 10,021 Crohn's disease 1,126,417 0 10,025 Crohn's disease 0 798,295 49 neg 54 10,026 Crohn's disease 221,962 511,471 10,027 Crohn's disease 1,339,734 84,567 10,028 Crohn's disease 0 0 10,031 Crohn's disease 4,173,769 0 10,033 Crohn's disease 937,019 173,028 108 neg 43 10,034 Crohn's disease 506,773 0 10,036 Crohn's disease 967,370 270,805 10,041 Crohn's disease 955,444 1,054,697 10,042 Crohn's disease 129,079 0 10,043 Crohn's disease 0 3,526,456 10,047 Crohn's disease — 281,080 10,058 Crohn's disease 2,812,932 259,435 9 neg 18 10,060 Crohn's disease — 175,351 10,061 Crohn's disease — 829,159 10,062 Crohn's disease 4,657,297 26,370 10,064 Crohn's disease 3,696,434 697,255 10,067 Crohn's disease — 1,478,295 10,068 Crohn's disease 23,386 800,316 10,071 Crohn's disease 2,627,120 711,760 10,073 Crohn's disease 6,178,401 1,576,664 72 neg 113 10,074 Crohn's disease 499,170 608,101 43 neg 18 10,075 Crohn's disease 5,264,056 2,824,132 10,077 Crohn's disease 417,861 302,912 15 neg 5 10,078 Crohn's disease 1,744,354 1,191,273 89 neg 104 10,081 Crohn's disease 4,236,189 471,166 39 neg 17 10,089 Crohn's disease 321,993 726,658 12 neg 29 10,090 Crohn's disease 736,699 161,181 10,094 Crohn's disease 997,472 954,849 10,095 Crohn's disease 1,187,706 720,792 10,102 Crohn's disease 131,432 475,933 10,051 No digestive disease 0 0 10,052 No digestive disease 339,032 0 10,053 No digestive disease 336,286 25,865 10,054 No digestive disease 1,014,501 0 10,059 Anal fissure 228,946 0 10,066 Proctitis/Psoriasis 844,179 97,181 10,080 No digestive disease 1,193,312 — 10,082 No digestive disease 1,678,410 — 10,003 Ulcerative colitis 361,718 258,224 10,020 Ulcerative colitis 541,282 — 10,022 Ulcerative colitis 2,348,965 0 40 neg 18 10,023 Ulcerative colitis 93,557 50,612 10,024 Ulcerative colitis 1,416,782 124,268 10,030 Ulcerative colitis 606,565 152,946 10,039 Ulcerative colitis 0 186,636 10,040 Ulcerative colitis 744,886 1,110,942 10,044 Ulcerative colitis 926,919 0 10,050 Ulcerative colitis 356,345 563,448 10,065 Ulcerative colitis 957,048 627,416 10,069 Ulcerative colitis 777,260 25,579 8 neg 8 10,072 Ulcerative colitis 268,387 96,768 10,079 Ulcerative colitis 432,879 0 10,084 Ulcerative colitis 112,154 101,127 10,086 Ulcerative colitis 598,760 47,781 10,087 Ulcerative colitis 110,275 108,383 6 neg 19 10,096 Ulcerative colitis 186,956 106,274 10,097 Ulcerative colitis 167,573 10,386 Cutoffs in percintiles 65 682,641 103,958 75 864,864 131,437 85 1,011,628 254,645 90 1,260,353 502,187 98 1,986,860 849,837 Avarage Crohn's disease 1,489,028 565,479 No Chrohn's Disease 618,517 136,809 Median Crohn's disease 937,019 302,912 No Chrohn's Disease 432,879 47,781 ttest CD vs Non CD 0.011365107 0.00374204

indicates data missing or illegible when filed

TABLE 3 Fluorescent signals from binding of IgA and IgM antibodies to different glycans in CD patients and non CD patients. Glycans are presented in LINEARCODE ®. Patient No. Clinical condition IgA GNb3ANa IgA GNb4GNb IgA Ma6Ma IgA Mannan IgM A[3S]b 10,001 Crohn's disease 0 0 0 2,471,242 0 10,004 Crohn's disease 0 31,779 0 7,528,498 0 10,005 Crohn's disease 0 338,274 0 1,227,302 0 10,006 Crohn's disease 23,445 11,875 76,964 946,221 0 10,007 Crohn's disease 0 0 0 917,187 0 10,008 Crohn's disease 103,139 174,915 62,386 1,310,822 0 10,009 Crohn's disease 0 0 0 92,347 0 10,011 Crohn's disease 0 346,998 172,408 2,711,283 0 10,012 Crohn's disease 152,601 228,391 96,406 588,862 0 10,013 Crohn's disease 398,314 244,995 74,172 152,447 1,274,136 10,015 Crohn's disease 276,400 662,981 207,563 805,291 387,307 10,016 Crohn's disease 282,558 60,337 59,441 2,633,452 280,897 10,018 Crohn's disease 0 0 0 394,458 248,996 10,021 Crohn's disease 42,493 0 0 335,624 762,735 10,025 Crohn's disease 316,210 0 118,211 1,399,632 466,121 10,026 Crohn's disease 93,421 0 5,028 199,707 0 10,027 Crohn's disease 68,671 16,665 2,855 642,630 151,387 10,028 Crohn's disease 8,000 101,278 21,251 775,450 510,204 10,031 Crohn's disease 17,959 7,639 171,149 1,043,614 165,011 10,033 Crohn's disease 130,546 0 143,284 2,808,678 14,440 10,034 Crohn's disease 28,933 76,677 0 796,415 770,101 10,036 Crohn's disease 166,203 638,663 313,056 894,349 163,646 10,041 Crohn's disease 72,138 312,400 40,870 1,134,466 123,889 10,042 Crohn's disease 121,281 461,961 96,621 448,555 0 10,043 Crohn's disease 609,401 946,450 175,316 2,132,133 0 10,047 Crohn's disease 130,239 89,417 354,536 3,330,642 831,510 10,058 Crohn's disease 110,420 127,556 83,918 705,563 128,555 10,060 Crohn's disease 492,266 0 1,680,299 4,655,963 363,862 10,061 Crohn's disease 158,347 610,869 308,553 1,513,948 690,511 10,062 Crohn's disease 289,501 687,427 334,870 6,413,459 715,200 10,064 Crohn's disease 539,120 966,435 359,881 7,316,963 245,487 10,067 Crohn's disease 35,566 72,174 67,348 853,714 222,329 10,068 Crohn's disease 512,740 619,837 152,275 646,134 0 10,071 Crohn's disease 697,219 227,591 264,323 2,120,939 67,858 10,073 Crohn's disease 1,108,947 1,190,028 275,651 4,724,418 0 10,074 Crohn's disease 102,766 151,414 0 2,834,813 0 10,075 Crohn's disease 0 0 0 2,892,894 65,267 10,077 Crohn's disease 432,092 229,195 0 448,889 0 10,078 Crohn's disease 410,956 328,642 922,740 2,545,334 0 10,081 Crohn's disease 371,099 165,461 35,119 1,313,553 0 10,089 Crohn's disease 939,597 1,113,730 277,224 515,127 0 10,090 Crohn's disease 302,186 226,019 54,115 2,362,390 0 10,094 Crohn's disease 497,476 190,609 45,591 435,905 68,165 10,095 Crohn's disease 10,806 2,644 0 507,917 0 10,102 Crohn's disease 46,151 18,064 0 343,762 0 10,051 No digestive disease 47,892 817,025 0 563,929 0 10,052 No digestive disease 117,302 150,206 93,166 605,430 0 10,053 No digestive disease 38,792 224,900 0 215,860 0 10,054 No digestive disease 99,132 97,152 97,283 744,642 0 10,059 Anal fissure 0 0 0 636,387 0 10,066 Proctitis/Psoriasis 422,665 580,445 0 1,448,060 255,814 10,080 No digestive disease 104,079 137,997 0 1,279,087 0 10,082 No digestive disease 1,071,426 53,617 17,586 833,228 0 10,003 Ulcerative colitis 8,237 26,105 0 823,317 0 10,020 Ulcerative colitis 0 0 0 524,406 0 10,022 Ulcerative colitis 0 0 0 739,593 0 10,023 Ulcerative colitis 259,476 0 0 685,296 0 10,024 Ulcerative colitis 0 0 24,598 639,507 0 10,030 Ulcerative colitis 0 0 76,523 1,016,276 0 10,039 Ulcerative colitis 48,020 11,750 0 846,823 0 10,040 Ulcerative colitis 0 0 0 831,587 0 10,044 Ulcerative colitis 11,952 83,214 18,860 413,193 7,575 10,050 Ulcerative colitis 51,955 5,070 0 360,267 0 10,065 Ulcerative colitis 0 101,517 0 464,560 646,009 10,069 Ulcerative colitis 9,590 0 38,568 419,600 0 10,072 Ulcerative colitis 7,293 0 15,179 999,491 0 10,079 Ulcerative colitis 0 0 35,661 603,017 0 10,084 Ulcerative colitis 0 0 0 428,114 0 10,086 Ulcerative colitis 0 0 0 607,351 16,235 10,087 Ulcerative colitis 262,818 0 12,060 873,344 175,021 10,096 Ulcerative colitis — — 56,446 371,917 — 10,097 Ulcerative colitis — — 40,759 571,976 — Avarage Crohn's disease 224,427 259,542 156,743 1,797,182 193,725 No Chrohn's Disease 94,838 84,778 19,507 686,898 40,765 Median Crohn's disease 121,281 151,414 67,348 1,043,614 14,440 No Chrohn's Disease 8,237 — — 636,387 — ttest CD vs Non CD 0.034732158 0.013016159 0.0156334 0.002570494 0.014433781 Patient No. Clinical condition IgMAa IgM Aa3Ab4GNb IgM Aa4Ab4Gb IgMAb3(GNb6)ANa IgM Ab3ANa 10,001 Crohn's disease 0 0 0 0 0 10,004 Crohn's disease 0 0 0 0 0 10,005 Crohn's disease 0 0 0 161,405 0 10,006 Crohn's disease 68,568 0 402,561 166,017 0 10,007 Crohn's disease 0 0 0 0 0 10,008 Crohn's disease 0 0 0 0 0 10,009 Crohn's disease 0 0 0 0 0 10,011 Crohn's disease 0 0 0 0 0 10,012 Crohn's disease 0 0 0 0 0 10,013 Crohn's disease 1,194,898 1,392,444 0 0 0 10,015 Crohn's disease 390,644 325,647 0 0 0 10,016 Crohn's disease 0 14,251 0 0 0 10,018 Crohn's disease 228,314 158,547 0 0 0 10,021 Crohn's disease 852,490 700,041 0 327,738 4,353 10,025 Crohn's disease 319,573 565,269 0 0 0 10,026 Crohn's disease 0 0 0 0 0 10,027 Crohn's disease 437,186 547,822 0 0 0 10,028 Crohn's disease 540,836 723,234 0 81,777 46,618 10,031 Crohn's disease 242,183 199,166 0 15,958 0 10,033 Crohn's disease 0 1,113,845 0 0 0 10,034 Crohn's disease 695,338 641,641 0 121,757 78,717 10,036 Crohn's disease 642,487 2,180,044 0 0 0 10,041 Crohn's disease 89,104 151,581 0 0 0 10,042 Crohn's disease 1,345,482 1,158,234 0 0 0 10,043 Crohn's disease 0 0 0 0 0 10,047 Crohn's disease 857,115 1,076,947 0 465,551 147,112 10,058 Crohn's disease 220,493 428,347 0 0 0 10,060 Crohn's disease 1,284,367 474,206 0 19,714 0 10,061 Crohn's disease 1,095,509 1,128,863 0 621,675 149,877 10,062 Crohn's disease 1,485,943 2,464,680 0 220,921 188,832 10,064 Crohn's disease 664,556 1,633,864 0 635,144 0 10,067 Crohn's disease 141,266 75,592 0 0 0 10,068 Crohn's disease 0 0 0 0 0 10,071 Crohn's disease 77,830 88,393 0 272,031 0 10,073 Crohn's disease 0 147,447 30,339 184,079 5,781 10,074 Crohn's disease 0 0 0 0 0 10,075 Crohn's disease 34,147 241,365 0 65,722 0 10,077 Crohn's disease 214,916 88,848 0 321,513 0 10,078 Crohn's disease 0 0 0 0 0 10,081 Crohn's disease 0 0 0 0 0 10,089 Crohn's disease 0 0 0 0 0 10,090 Crohn's disease 0 0 0 6,781 0 10,094 Crohn's disease 118,072 97,315 93,394 70,477 4,567 10,095 Crohn's disease 0 0 0 0 0 10,102 Crohn's disease 0 0 0 0 0 10,051 No digestive disease 0 0 0 0 0 10,052 No digestive disease 0 0 0 0 0 10,053 No digestive disease 803,031 314,948 0 0 0 10,054 No digestive disease 0 33,896 86,151 118,374 0 10,059 Anal fissure 0 0 47,680 0 0 10,066 Proctitis/Psoriasis 613,172 366,385 711,585 672,896 648,481 10,080 No digestive disease 142,813 1,437,670 357,136 255,635 13,016 10,082 No digestive disease 0 0 361,634 265,794 58,052 10,003 Ulcerative colitis 0 0 0 0 0 10,020 Ulcerative colitis 0 0 0 0 0 10,022 Ulcerative colitis 0 230,936 5,354 0 0 10,023 Ulcerative colitis 0 0 538,071 0 0 10,024 Ulcerative colitis 0 0 119,591 2,544,871 64,642 10,030 Ulcerative colitis 0 0 0 79,886 60,684 10,039 Ulcerative colitis 0 0 0 26,754 32,355 10,040 Ulcerative colitis 0 0 0 0 0 10,044 Ulcerative colitis 99,214 210,443 53,421 548,458 59,039 10,050 Ulcerative colitis 0 0 0 0 0 10,065 Ulcerative colitis 651,393 679,823 669,033 381,610 201,783 10,069 Ulcerative colitis 0 0 0 0 0 10,072 Ulcerative colitis 0 0 0 375,381 0 10,079 Ulcerative colitis 79,891 36,805 512,305 182,972 79,189 10,084 Ulcerative colitis 0 0 0 0 0 10,086 Ulcerative colitis 221,934 0 293,278 0 0 10,087 Ulcerative colitis 175,678 321,514 145,748 337,665 220,927 10,096 Ulcerative colitis — — — — — 10,097 Ulcerative colitis — — — 1,022,582 33,778 Avarage Crohn's disease 294,251 395,947 11,695 83,517 13,908 No Chrohn's Disease 103,227 134,534 144,481 252,329 54,516 Median Crohn's disease 68,568 88,848 — — — No Chrohn's Disease — — — — — ttest CD vs Non CD 0.036400897 0.041051138 0.000390828 0.047775166 0.059924749 Patient No. Clinical condition IgM GNb3Ab4Gb IgM Gab3ANa IgM Dextran IgM Mannan 10,001 Crohn's disease 608,457 657,592 230,160 2,084,216 10,004 Crohn's disease 542,436 730,879 360,390 722,969 10,005 Crohn's disease 1,375,070 2,933,445 388,048 117,931 10,006 Crohn's disease 1,695,894 1,963,416 3,110,617 810,502 10,007 Crohn's disease 1,178,646 993,402 969,884 612,220 10,008 Crohn's disease 735,201 1,372,932 468,089 796,727 10,009 Crohn's disease 322,451 680,970 59,317 0 10,011 Crohn's disease 205,150 1,039,057 494,848 0 10,012 Crohn's disease 972,528 1,688,071 1,221,314 1,490,590 10,013 Crohn's disease 1,174,565 2,149,276 2,049,830 2,588,785 10,015 Crohn's disease 1,054,492 1,545,327 2,107,182 1,418,006 10,016 Crohn's disease 1,328,058 3,127,245 1,667,531 70,238 10,018 Crohn's disease 1,430,548 1,912,812 2,166,938 1,971,045 10,021 Crohn's disease 1,469,658 2,142,999 2,094,545 1,285,376 10,025 Crohn's disease 2,975,994 3,777,466 2,312,303 2,435,341 10,026 Crohn's disease 745,061 3,384,432 3,509,606 409,591 10,027 Crohn's disease 1,779,829 3,425,917 1,720,479 3,144,625 10,028 Crohn's disease 1,562,488 2,857,289 2,236,531 3,049,138 10,031 Crohn's disease 578,140 849,394 973,540 910,393 10,033 Crohn's disease 879,981 1,786,910 822,422 587,545 10,034 Crohn's disease 1,117,458 1,646,969 1,616,572 1,320,077 10,036 Crohn's disease 2,573,605 2,518,175 2,570,459 1,552,108 10,041 Crohn's disease 781,745 1,733,620 929,763 1,789,860 10,042 Crohn's disease 2,298,533 3,652,328 4,851,471 1,342,805 10,043 Crohn's disease 943,254 3,801,228 349,534 628,039 10,047 Crohn's disease 1,854,934 3,495,774 3,233,236 1,936,982 10,058 Crohn's disease 897,206 1,775,488 766,028 983,376 10,060 Crohn's disease 926,098 2,910,549 1,296,914 859,571 10,061 Crohn's disease 2,612,378 3,589,958 2,379,098 4,685,631 10,062 Crohn's disease 1,464,405 2,716,333 1,256,919 1,245,680 10,064 Crohn's disease 1,893,522 3,343,233 2,212,175 2,923,034 10,067 Crohn's disease 631,443 1,765,852 1,280,499 1,011,954 10,068 Crohn's disease 0 829,715 0 0 10,071 Crohn's disease 669,203 1,023,200 302,307 2,573,082 10,073 Crohn's disease 693,896 1,180,873 1,506,812 2,148,575 10,074 Crohn's disease 1,549,121 2,082,866 1,385,468 531,246 10,075 Crohn's disease 839,403 1,814,627 1,571,440 582,384 10,077 Crohn's disease 576,897 1,309,189 1,059,111 359,244 10,078 Crohn's disease 43,955 952,620 464,210 791,441 10,081 Crohn's disease 28,386 907,289 0 93,410 10,089 Crohn's disease 0 319,608 309,448 0 10,090 Crohn's disease 326,922 551,737 253,387 635,071 10,094 Crohn's disease 427,222 634,599 741,618 1,331,605 10,095 Crohn's disease 331,811 1,357,109 711,417 2,803,494 10,102 Crohn's disease 0 78,782 0 860,022 10,051 No digestive disease 0 0 70,898 0 10,052 No digestive disease 0 0 0 0 10,053 No digestive disease 909,819 1,957,899 2,693,514 160,549 10,054 No digestive disease 1,417,516 2,034,746 1,190,821 0 10,059 Anal fissure 621,074 860,869 553,501 0 10,066 Proctitis/Psoriasis 1,399,015 2,112,942 2,036,326 2,851,646 10,080 No digestive disease 682,951 1,753,399 466,998 226,598 10,082 No digestive disease 1,059,304 3,803,965 460,919 0 10,003 Ulcerative colitis 0 0 0 0 10,020 Ulcerative colitis 0 0 0 0 10,022 Ulcerative colitis 530,365 1,220,301 910,810 0 10,023 Ulcerative colitis 345,812 630,728 401,625 107,462 10,024 Ulcerative colitis 1,271,762 2,775,365 877,756 1,161,681 10,030 Ulcerative colitis 606,210 622,605 1,558,797 0 10,039 Ulcerative colitis 732,277 910,699 542,797 51,325 10,040 Ulcerative colitis 798,350 1,289,960 751,879 156,868 10,044 Ulcerative colitis 402,433 873,504 961,761 697,203 10,050 Ulcerative colitis 445,848 728,257 737,999 134,037 10,065 Ulcerative colitis 1,203,436 1,975,174 1,363,891 1,042,417 10,069 Ulcerative colitis 1,127,666 1,331,796 0 313,657 10,072 Ulcerative colitis 825,856 1,084,765 2,081,853 556,681 10,079 Ulcerative colitis 828,413 1,477,648 992,691 696,436 10,084 Ulcerative colitis 650,663 1,075,158 309,983 489,824 10,086 Ulcerative colitis 835,147 1,931,046 1,405,512 824,630 10,087 Ulcerative colitis 592,753 1,540,709 897,343 1,016,898 10,096 Ulcerative colitis 331,000 441,228 452,374 582,057 10,097 Ulcerative colitis 854,116 2,517,741 913,480 517,995 Avarage Crohn's disease 1,024,356 1,895,124 1,333,594 1,277,643 No Chrohn's Disease 684,140 1,294,463 838,279 429,258 Median Crohn's disease 897,206 1,765,852 1,221,314 983,376 No Chrohn's Disease 682,951 1,220,301 751,879 160,549 ttest CD vs Non CD 0.029702213 0.017590683 0.034573847 0.000253081

TABLE 4 Specificity and sensitivity of the different IgG anti glycans for differentiation between CD and other digestive diseases using different cut-off values. The cutoff values for each glycans where set as the 89 percentiles of the other digestive disease group. Glycans are presented in LINEARCODE ®. Anti Glycan IgG antibodies Cut-off level Gb Gb3Gb Gb4Gb GGN[6S]b MMa Ma3(Ma6)Mb MMa3Ma MMannan XXylan MMa2Ma 65 percentile of Sensitivity for 76 73 62 62 58 60 71 78 58 71 non CD CD % Specificity for 70 63 67 70 67 78 70 67 63 67 CD % 75 percentile of Sensitivity for 62 71 58 60 71 60 64 73 62 71 non CD CD % Specificity for 74 74 78 78 52 78 78 74 74 78 CD % 85 percentile of Sensitivity for 56 64 51 49 49 56 62 67 40 62 non CD CD % Specificity for 81 81 81 81 85 85 81 81 81 85 CD % 90 percentile of Sensitivity for 49 62 33 42 44 56 60 47 36 40 non CD CD % Specificity for 89 89 89 89 89 93 89 89 89 89 CD %

TABLE 5 The sensitivity, specificity, True Positives (TP), True Negative (TN), False Positives (FP), and False Negatives (FN), and Positive Predictive Value (PPV) in different cut-of values for differentiation between CD and other digestive disease according to the level of Anti-Glc (β 1-3) Glc (β) IgG. IgG Gb3Gb (abnormals above cut-off) Sensitivity Specificity TP TN FP FN PPV — 100.0% 0.0% 45 0 27 0 62.5 0 95.6% 0.0% 43 0 27 2 61.43 6,963 95.6% 3.7% 43 1 26 2 62.32 27,235 93.3% 3.7% 42 1 26 3 61.76 27,402 91.1% 3.7% 41 1 26 4 61.19 62,662 88.9% 3.7% 40 1 26 5 60.61 78,554 88.9% 7.4% 40 2 25 5 61.54 86,949 88.9% 11.1% 40 3 24 5 62.5 87,267 86.7% 11.1% 39 3 24 6 61.9 108,535 86.7% 14.8% 39 4 23 6 62.9 133,683 86.7% 18.5% 39 5 22 6 63.93 156,547 86.7% 22.2% 39 6 21 6 65 174,695 86.7% 25.9% 39 7 20 6 66.1 241,622 84.4% 25.9% 38 7 20 7 65.52 242,565 84.4% 29.6% 38 8 19 7 66.67 312,940 84.4% 33.3% 38 9 18 7 67.86 317,476 84.4% 37.0% 38 10 17 7 69.09 344,750 82.2% 37.0% 37 10 17 8 68.52 371,507 80.0% 37.0% 36 10 17 9 67.92 371,648 80.0% 40.7% 36 11 16 9 69.23 378,722 77.8% 40.7% 35 11 16 10 68.63 379,003 77.8% 44.4% 35 12 15 10 70 430,129 77.8% 48.1% 35 13 14 10 71.43 441,239 77.8% 51.9% 35 14 13 10 72.92 454,736 75.6% 51.9% 34 14 13 11 72.34 489,733 75.6% 55.6% 34 15 12 11 73.91 525,203 73.3% 55.6% 33 15 12 12 73.33 526,443 73.3% 59.3% 33 16 11 12 75 546,432 73.3% 63.0% 33 17 10 12 76.74 612,367 73.3% 66.7% 33 18 9 12 78.57 851,962 73.3% 70.4% 33 19 8 12 80.49 979,509 71.1% 70.4% 32 19 8 13 80 1,209,892 71.1% 74.1% 32 20 7 13 82.05 1,266,954 71.1% 77.8% 32 21 6 13 84.21 1,317,083 68.9% 77.8% 31 21 6 14 83.78 1,376,957 66.7% 77.8% 30 21 6 15 83.33 1,379,223 66.7% 81.5% 30 22 5 15 85.71 1,425,185 64.4% 81.5% 29 22 5 16 85.29 1,461,919 64.4% 85.2% 29 23 4 16 87.88 1,560,904 62.2% 85.2% 28 23 4 17 87.5 1,574,353 62.2% 88.9% 28 24 3 17 90.32 1,705,604 60.0% 88.9% 27 24 3 18 90 1,722,429 57.8% 88.9% 26 24 3 19 89.66 1,732,725 57.8% 92.6% 26 25 2 19 92.86 1,817,947 55.6% 92.6% 25 25 2 20 92.59 1,825,768 53.3% 92.6% 24 25 2 21 92.31 1,869,774 51.1% 92.6% 23 25 2 22 92 1,880,984 48.9% 92.6% 22 25 2 23 91.67 1,994,899 46.7% 92.6% 21 25 2 24 91.3 2,067,775 44.4% 92.6% 20 25 2 25 90.91 2,154,175 42.2% 92.6% 19 25 2 26 90.48 2,324,221 40.0% 92.6% 18 25 2 27 90 2,467,429 37.8% 92.6% 17 25 2 28 89.47 2,551,776 35.6% 92.6% 16 25 2 29 88.89 2,703,085 35.6% 96.3% 16 26 1 29 94.12 2,850,072 33.3% 96.3% 15 26 1 30 93.75 3,096,078 31.1% 96.3% 14 26 1 31 93.33 3,186,273 28.9% 96.3% 13 26 1 32 92.86 3,441,678 28.9% 100.0% 13 27 0 32 100 3,511,076 26.7% 100.0% 12 27 0 33 100 3,559,430 24.4% 100.0% 11 27 0 34 100 3,578,889 22.2% 100.0% 10 27 0 35 100 4,137,076 20.0% 100.0% 9 27 0 36 100 4,327,530 17.8% 100.0% 8 27 0 37 100 5,107,549 15.6% 100.0% 7 27 0 38 100 5,545,432 13.3% 100.0% 6 27 0 39 100 5,640,050 11.1% 100.0% 5 27 0 40 100 5,724,798 8.9% 100.0% 4 27 0 41 100 6,708,583 6.7% 100.0% 3 27 0 42 100 6,891,638 4.4% 100.0% 2 27 0 43 100 7,209,245 2.2% 100.0% 1 27 0 44 100 7,299,442 0.0% 100.0% 0 27 0 45 ##### 

1. A method of diagnosing Crohn's disease in a subject, the method comprising providing a test sample from a subject; and determining whether at least one an anti-glycan antibody is present in said sample, wherein said at least one anti-glycan antibody is selected from the group consisting of an anti-Glc (β) antibody, an anti-Glc (β 1-4) Glc (β) antibody, an anti-Glc (β 1-3) Glc (β) antibody, an anti-GlcNAc 6-sulfate (β) antibody an anti-Dextran antibody, an anti-Xylan antibody, an anti-GlcNAc (β 1-4) GlcNAc (β) antibody, an anti-Gal 3 sulphate (β) antibody, an anti-GlcNAc (β 1-3) GalNAc (β) antibody, an anti-GlcNAc (β 1-3) Gal (β 1-4) Glc (β) antibody, and an anti-Gal (α 1-3) Gal (β 1-4) GlcNAc (β) antibody; wherein the presence of said antibody in said test sample indicates the subject has Crohn's disease.
 2. The method of claim 1, wherein said method further comprises comparing the levels of said at least one anti-glycan antibody in said test sample to the levels of said at least one anti-glycan antibody in a control sample, wherein said control sample is selected from the group consisting of one or more individuals known to have or not to have a gastrointestinal disorder other than Crohn's disease.
 3. The method of claim 2, wherein said control sample is from one or more individuals with a gastrointestinal disorder that is irritable bowel syndrome or ulcerative colitis.
 4. The method of claim 2, wherein said control sample is from one or more individuals that do not have a gastrointestinal disorder.
 5. The method of claim 1, wherein said method comprises detecting at least two of said antibodies.
 6. The method of claim 1, wherein said method comprises detecting at least four of said antibodies.
 7. The method of claim 1, wherein said method comprises detecting at least six of said antibodies.
 8. The method of claim 1, further comprising determining whether said test sample has an anti-Mannan (ASCA) antibody, wherein the presence of said anti-ASCA antibody in said sample indicates the subject has Crohn's Disease.
 9. The method of claim 1, further comprising determining whether said test sample has an anti-neutrophil cytoplasmic antibodies (ANCA), wherein the presence of said anti-neutrophil cytoplasmic antibodies (ANCA) indicates said subject does not have Crohn's Disease.
 10. The method of claim 8, further comprising determining whether said test sample has an anti-neutrophil cytoplasmic antibodies (ANCA), wherein the presence of said anti-neutrophil cytoplasmic antibodies (ANCA) indicates said subject does not have Crohn's Disease.
 11. The method of claim 1, wherein said method comprises detecting said anti-Glc (β 1-3) Glc (β) antibody, and one, two, or three of said anti-Man (α 1-3) Man (α) antibody, anti-Man (α 1-3)[Man (α 1-6)] Man (α) antibody, anti-Man (α 1-2) Man (α), anti-Man (α 1-6) Man (α) or an anti-Mannan (ASCA) antibody.
 12. The method of claim 1, wherein said test sample is a biological fluid.
 13. The method of claim 12, wherein said biological fluid is whole blood, serum, plasma, urine, or saliva.
 14. The method of claim 11, wherein said wherein said biological fluid is serum.
 15. The method of claim 1, further comprising determining the isotype of said antibody.
 16. The method of claim 15, wherein said at least one antibody is an IgM type antibody.
 17. The method of claim 15, wherein said at least one antibody is an IgA type antibody.
 18. The method of claim 15, wherein said at least one antibody is an IgG type antibody.
 19. The method of claim 18, wherein said IgG antibody is an anti-Glc (β) antibody, an anti-Glc (β 1-3) Glc (β) antibody, an anti-Glc (β 1-4) Glc (β) antibody, an anti-GlcNAc (β) 6-sulfate antibody, or an anti-Xylan antibody.
 20. The method of claim 1, wherein said at least one anti-glycan antibody is detected using a fluorescent antibody.
 21. The method of claim 1, wherein said at least one anti-glycan antibody is detected using an enzyme-linked immunoabsorbent assay (ELISA).
 22. A method of diagnosing Crohn's disease in a subject, the method comprising providing a test sample from a subject; and determining whether an anti-glycan antibody is present in said test sample, wherein said at least one anti-glycan antibody is selected from the group consisting of an IgG Glc (β 1-3) Glc (β) antibody and an IgG anti-Man (α 1-3) Man (α) antibody, wherein the presence of said at least one antibody in said test sample indicates the subject has Crohn's disease.
 23. The method of claim 22, wherein said method comprises detecting an IgG anti-Glc (β 1-3) Glc (β) antibody.
 24. The method of claim 23, wherein said method comprises detecting an IgG anti-Man (α 1-3) Man (α) antibody.
 25. The method of claim 22, wherein said method comprises detecting an IgG Glc (β 1-3) Glc (β) antibody and an IgG anti-Man (α 1-3) Man (α) antibody.
 26. The method of claim 22, wherein said method further comprises determining whether said sample has an IgG anti-Mannan or an IgA anti-Mannan antibody, wherein the presence of said IgG anti-Mannan or IgA anti-Mannan antibody in said sample indicates said subject has Crohn's disease.
 27. The method of claim 26, wherein said method comprises determining whether said sample has an IgG anti-Mannan antibody.
 28. The method of claim 26, wherein said method comprises determining whether said sample has an IgA anti-Mannan antibody.
 29. The method of claim 26, wherein said method further comprises determining whether said sample has an anti-neutrophil cytoplasmic antibodies (ANCA), wherein the absence of said antibody in said sample indicates said subject has Crohn's disease.
 30. A method of differentially diagnosing Crohn's disease or inflammatory bowel disease in a subject, the method comprising providing a test sample from a subject; and determining whether said antibodies are present in said sample, said antibodies comprising anti-neutrophil cytoplasmic antibody (ANCA), IgG anti-Glc (β 1-3) Glc (β) IgG ASCA; and IgA ASCA, wherein the absence of ANCA and the presence of at least one of said IgG anti-Glc (β 1-3) Glc (β) IgG ASCA, and IgA ASCA antibodies in said test sample indicates the subject has Crohn's disease, and wherein the presence of at least one of said antibodies in said test sample indicates the subject has inflammatory bowel disease (UC or CD).
 31. A kit for diagnosing Crohn's Disease, the kit comprising one or more reagents that specifically detect an antibody selected from the group consisting of an anti-Glc (β) antibody, an anti-Glc (β 1-4) Glc (β) antibody, an anti-Glc (β 1-3) Glc (β) antibody, an anti-GlcNAc 6-sulfate (β) antibody, an anti-Man (α 1-2) Man (α) antibody, an anti-Man (α 1-3) Man (α) antibody, an anti-Man (α 1-6) Man (α) antibody, an anti-Man (α) antibody, an anti-Man (α 1-3)[Man (α 1-6)] Man (α), an anti-Dextran antibody, an anti-Xylan antibody, an anti-GlcNAc (β 1-4) GlcNAc (β) antibody, an anti-Gal 3 sulphate (β) antibody, an anti-aGlcNAc (β 1-3) GalNAc (β) antibody, an anti-GlcNAc (β 1-3) Gal (β 1-4) Glc (β) antibody, and an anti-Gal (α 1-3) Gal (β 1-4) GlcNAc (β) antibody.
 32. The kit of claim 31, further including instructions for using said kit.
 33. The kit of claim 31, wherein said kit further comprises one or more reagents that specifically detect an anti-Mannan (ASCA) antibodies or anti-neutrophil cytoplasmic antibodies (ANCA).
 34. The kit of claim 31, wherein said kit comprises a reagent or reagents that specifically detects an anti-Mannan (ASCA) antibody and a anti-neutrophil cytoplasmic antibodies (ANCA).
 35. The kit of claim 31, further comprising a reagent that specifically detects an IgG, IgM, or IgA-type antibody.
 36. The kit of claim 31, wherein said reagents are provided on an array.
 37. An array comprising a plurality of carbohydrate reagents that specifically detect an antibody selected from the group consisting of an anti-Glc (β) antibody, an anti-Glc (β1-4) Glc (β) antibody, an anti-Glc (β 1-3) Glc (β) antibody, an anti-GlcNAc 6-sulfate (β) antibody, an anti-Man (α 1-2) Man (α) antibody, an anti-Man (α 1-3) Man (α) antibody, an anti-Man (α 1-6) Man (α) antibody, an anti-Man (α) antibody, an anti-Man (α 1-3)[Man (α 1-6)] Man (α), an anti-Dextran antibody, an anti-Xylan antibody, an anti-GlcNAc (β 1-4) GlcNAc (β) antibody, an anti-Gal 3 sulphate (β) antibody, an anti-aGlcNAc (β 1-3) GalNAc (β) antibody, an anti-GlcNAc (β 3-3) Gal (β 1-4) Glc (β) antibody, and an anti-Gal (α 1-3) Gal (β 1-4) GlcNAc (β) antibody, wherein said reagents are attached at an addressable location on said array.
 38. The array of claim 37, further comprising a carbohydrate reagent or reagents that detect an anti-Mannan (ASCA) antibody or a anti-neutrophil cytoplasmic antibodies (ANCA).
 39. The array of claim 38, wherein said array comprises carbohydrate reagent or reagents that detect an anti-Mannan (ASCA) antibody and anti-neutrophil cytoplasmic antibodies (ANCA).
 40. The array of claim 37, wherein each of said glycans are attached to said array via a linker.
 41. The array of claim 40, wherein said linker includes at least one ethylene glycol derivative, at least two cyanuric chloride derivatives and an anilino group.
 42. The array of claim 37, wherein at least two of said reagent or reagents are provided at the same location on said addressable array. 